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Part Four: Appendix B: Mathematical Basics

Part Four
Appendix B: Mathematical Basics
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table of contents
  1. Chapter 25
    1. Anatomy of the Circulatory and Lymphatic Systems
      1. The Lymphatic System
      2. Infections of the Circulatory System
      3. Infections of the Lymphatic System
    2. Bacterial Infections of the Circulatory and Lymphatic Systems
      1. Puerperal Sepsis
      2. Infectious Arthritis
      3. Osteomyelitis
      4. Rheumatic Fever
      5. Bacterial Endocarditis and Pericarditis
      6. Gas Gangrene
      7. Tularemia
      8. Brucellosis
      9. Cat-Scratch Disease
      10. Rat-Bite Fever
      11. Plague
      12. Zoonotic Febrile Diseases
    3. Viral Infections of the Circulatory and Lymphatic Systems
      1. Cytomegalovirus Infections
      2. Arthropod-Borne Viral Diseases
      3. Ebola Virus Disease
      4. Hantavirus
      5. Human Immunodeficiency Virus
    4. Parasitic Infections of the Circulatory and Lymphatic Systems
      1. Toxoplasmosis
      2. Babesiosis
      3. Chagas Disease
      4. Leishmaniasis
      5. Schistosomiasis
      6. Summary
      7. Review Questions
  2. Chapter 26
    1. Anatomy of the Nervous System
      1. The Peripheral Nervous System
      2. The Cells of the Nervous System
      3. Meningitis and Encephalitis
    2. Bacterial Diseases of the Nervous System
      1. Hansen’s Disease (Leprosy)
    3. Acellular Diseases of the Nervous System
      1. Zika Virus Infection
      2. Rabies
      3. Poliomyelitis
      4. Transmissible Spongiform Encephalopathies
    4. Fungal and Parasitic Diseases of the Nervous System
      1. Amoebic Meningitis
      2. Granulomatous Amoebic Encephalitis
      3. Human African Trypanosomiasis
      4. Neurotoxoplasmosis
      5. Neurocysticercosis
      6. Summary
      7. Review Questions
  3. Chapter 11
    1. The Functions of Genetic Material
    2. DNA Replication
      1. DNA Replication in Eukaryotes
      2. DNA Replication of Extrachromosomal Elements: Plasmids and Viruses
    3. RNA Transcription
      1. Transcription in Eukaryotes
    4. Protein Synthesis (Translation)
      1. The Protein Synthesis Machinery
      2. The Mechanism of Protein Synthesis
      3. Protein Targeting, Folding, and Modification
    5. Mutations
      1. Effects of Mutations on Protein Structure and Function
      2. Causes of Mutations
      3. DNA Repair
      4. Identifying Bacterial Mutants
      5. The Ames Test
    6. How Asexual Prokaryotes Achieve Genetic Diversity
      1. Transduction
      2. Conjugation
      3. Transposition
    7. Gene Regulation: Operon Theory
      1. Regulation by Repression
      2. Global Responses of Prokaryotes
      3. Additional Methods of Regulation in Bacteria: Attenuation and Riboswitches
      4. Other Factors Affecting Gene Expression in Prokaryotes and Eukaryotes
      5. Summary
      6. Review Questions
  4. Appendix A: Fundamentals of Physics and Chemistry
    1. Atomic Structure
    2. Chemical Elements
    3. Chemical Bonds
    4. Molecular Formula, Molecular Mass, and the Mole
    5. Energy
    6. Chemical Reactions
    7. Acids and Bases
  5. Appendix B: Mathematical Basics
    1. Calculating Percents
    2. Multiplying and Dividing by Tens
    3. Scientific Notation
    4. Expressing Numbers in Scientific Notation
    5. Logarithms
    6. Rounding and Significant Digits
    7. Generation Time
    8. Most Probable Number
  6. Appendix C: Glossary

Appendix B: Mathematical Basics

Squares and Other Powers

An exponent, or a power, is mathematical shorthand for repeated multiplications. For example, the exponent “2” means to multiply the base for that exponent by itself (in the example here, the base is “5”):

52 = 5 × 5 = 25

The exponent is “2” and the base is the number “5.” This expression (multiplying a number by itself) is also called a square. Any number raised to the power of 2 is being squared. Any number raised to the power of 3 is being cubed:

53 = 5 × 5 × 5 = 125

A number raised to the fourth power is equal to that number multiplied by itself four times, and so on for higher powers. In general:

Calculating Percents

nx = n × nx − 1

A percent is a way of expressing a fractional amount of something using a whole divided into 100 parts. A percent is

a ratio whose denominator is 100. We use the percent symbol, %, to show percent. Thus, 25% means a ratio of 25 ,

100

3% means a ratio of 3 , and 100 % percent means 100 , or a whole.

100 100

Converting Percents

A percent can be converted to a fraction by writing the value of the percent as a fraction with a denominator of 100 and simplifying the fraction if possible.

25% = 25 1

4

[TextboxStart]=

[TextboxStart]100

A percent can be converted to a decimal by writing the value of the percent as a fraction with a denominator of 100 and dividing the numerator by the denominator.

10% = 10

100


= 0.10

To convert a decimal to a percent, write the decimal as a fraction. If the denominator of the fraction is not 100, convert it to a fraction with a denominator of 100, and then write the fraction as a percent.

0.833 = 833 = 83.3 = 83.3%

100

[TextboxStart]1000

To convert a fraction to a percent, first convert the fraction to a decimal, and then convert the decimal to a percent.

3 = 0.75 = 75

= 75%

4 100

Suppose a researcher finds that 15 out of 23 students in a class are carriers of Neisseria meningitides. What percentage of students are carriers? To find this value, first express the numbers as a fraction.

carriers = 15

total students 23

Then divide the numerator by the denominator.

15 = 15 ÷ 23 ≈ 0.65

23

Finally, to convert a decimal to a percent, multiply by 100.

0.65 × 100 = 65%

The percent of students who are carriers is 65%.

You might also get data on occurrence and non-occurrence; for example, in a sample of students, 9 tested positive for Toxoplasma antibodies, while 28 tested negative. What is the percentage of seropositive students? The first step is to determine the “whole,” of which the positive students are a part. To do this, sum the positive and negative tests.

positive + negative = 9 + 28 = 37

The whole sample consisted of 37 students. The fraction of positives is:

positive = 9

total students 37

To find the percent of students who are carriers, divide the numerator by the denominator and multiply by 100.

The percent of positive students is about 24%.

9 = 9 ÷ 37 ≈ 0.24

0.24 × 100 = 24%

37

Another way to think about calculating a percent is to set up equivalent fractions, one of which is a fraction with 100 as the denominator, and cross-multiply. The previous example would be expressed as:

Now, cross multiply and solve for the unknown:

9 × 100 = 37x

9 x

37 100

=

9 × 100

37

900

37

= x Divide both sides by 37

= x Multiply

The answer, rounded, is the same.

24 ≈ x Divide

Multiplying and Dividing by Tens

In many fields, especially in the sciences, it is common to multiply decimals by powers of 10. Let’s see what happens when we multiply 1.9436 by some powers of 10.

1.9436(10) = 19.436

1.9436(100) = 194.36

1.9436(1000) = 1943.6

The number of places that the decimal point moves is the same as the number of zeros in the power of ten. Table B1

summarizes the results.

Multiply by Zeros Decimal point moves . . .

10

1

1 place to the right
100

2

2 places to the right
1,000

3

3 places to the right
10,000

4

4 places to the right

Table B1

We can use this pattern as a shortcut to multiply by powers of ten instead of multiplying using the vertical format. We can count the zeros in the power of 10 and then move the decimal point that same number of places to the right.

So, for example, to multiply 45.86 by 100, move the decimal point 2 places to the right.

Sometimes when we need to move the decimal point, there are not enough decimal places. In that case, we use zeros as placeholders. For example, let’s multiply 2.4 by 100. We need to move the decimal point 2 places to the right. Since there is only one digit to the right of the decimal point, we must write a 0 in the hundredths place.

When dividing by powers of 10, simply take the opposite approach and move the decimal to the left by the number of zeros in the power of ten.

Let’s see what happens when we divide 1.9436 by some powers of 10.

1.9436 ÷ 10 = 0.19436

1.9436 ÷ 100 = 0.019436

1.9436 ÷ 1000 = 0.0019436

If there are insufficient digits to move the decimal, add zeroes to create places.

Scientific Notation

Scientific notation is used to express very large and very small numbers as a product of two numbers. The first number of the product, the digit term, is usually a number not less than 1 and not greater than 10. The second number of the product, the exponential term, is written as 10 with an exponent. Some examples of scientific notation are given in Table B2.

Standard Notation Scientific Notation

1000

1 × 103

100

1 × 102

10

1 × 101

1

1 × 100

0.1

1 × 10−1

0.01

1 × 10−2

Table B2

Scientific notation is particularly useful notation for very large and very small numbers, such as 1,230,000,000 = 1.23

× 109, and 0.00000000036 = 3.6 × 10−10.

Expressing Numbers in Scientific Notation

Converting any number to scientific notation is straightforward. Count the number of places needed to move the decimal next to the left-most non-zero digit: that is, to make the number between 1 and 10. Then multiply that number by 10 raised to the number of places you moved the decimal. The exponent is positive if you moved the decimal to the left and negative if you moved the decimal to the right. So

and

2386 = 2.386 × 1000 = 2.386 × 103

0.123 = 1.23 × 0.1 = 1.23 × 10−1

The power (exponent) of 10 is equal to the number of places the decimal is shifted.

Logarithms

The common logarithm (log) of a number is the power to which 10 must be raised to equal that number. For example, the common logarithm of 100 is 2, because 10 must be raised to the second power to equal 100. Additional examples are in Table B3.

Number Exponential Form Common Logarithm

1000

103

3

10

101

1

1

100

0

0.1

10−1

−1

0.001

10−3

−3

Table B3

To find the common logarithm of most numbers, you will need to use the LOG button on a calculator.

Rounding and Significant Digits

In reporting numerical data obtained via measurements, we use only as many significant figures as the accuracy of the measurement warrants. For example, suppose a microbiologist using an automated cell counter determines that there are 525,341 bacterial cells in a one-liter sample of river water. However, she records the concentration as 525,000 cells per liter and uses this rounded number to estimate the number of cells that would likely be found in 10 liters of river water. In this instance, the last three digits of the measured quantity are not considered significant. They are rounded to account for variations in the number of cells that would likely occur if more samples were measured.

The importance of significant figures lies in their application to fundamental computation. In addition and subtraction, the sum or difference should contain as many digits to the right of the decimal as that in the least certain (indicated by underscoring in the following example) of the numbers used in the computation.

Suppose a microbiologist wishes to calculate the total mass of two samples of agar.

4.38 _3 g 3.002 1_ g

7.38 _5 g

The least certain of the two masses has three decimal places, so the sum must have three decimal places.

In multiplication and division, the product or quotient should contain no more digits than than in the factor containing the least number of significant figures. Suppose the microbiologist would like to calculate how much of a reagent would be present in 6.6 mL if the concentration is 0.638 g/mL.

0.63 _8 g × 6. _6 mL = 4.1 g

mL

Again, the answer has only one decimal place because this is the accuracy of the least accurate number in the calculation.

When rounding numbers, increase the retained digit by 1 if it is followed by a number larger than 5 (“round up”). Do not change the retained digit if the digits that follow are less than 5 (“round down”). If the retained digit is followed by 5, round up if the retained digit is odd, or round down if it is even (after rounding, the retained digit will thus always be even).

Generation Time

It is possible to write an equation to calculate the cell numbers at any time if the number of starting cells and doubling time are known, as long as the cells are dividing at a constant rate. We define N0 as the starting number of bacteria, the number at time t = 0. Ni is the number of bacteria at time t = i, an arbitrary time in the future. Finally we will set j equal to the number of generations, or the number of times the cell population doubles during the time interval. Then we have,

Ni = N0 × 2 j

This equation is an expression of growth by binary fission.

In our example, N0 = 4, the number of generations, j, is equal to 3 after 90 minutes because the generation time is 30 minutes. The number of cells can be estimated from the following equation:

Ni = N0 × 2 j

N90 = 4 × 23

N90 = 4 × 8 = 32

The number of cells after 90 minutes is 32.

Most Probable Number

The table in Figure B1 contains values used to calculate the most probable number example given in How Microbes Grow.

Figure B1

Annotate

Next Chapter
Appendix C: Glossary
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Microbiology for Allied Health Students
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